Researchers from the University of Bristol and NHS Blood and Transplant have generated the first immortalised cell lines which allow for more efficient manufacture of red blood cells.
They were able to manufacture red blood cells in a more efficient scale than was previously possible.
The results, published in Nature Communications, could, if successfully tested in clinical trials, eventually lead to a safe source of transfusions for people with rare blood types, and in areas of the world where blood supplies are inadequate or unsafe.
Previously, research in this field focused on growing donated stem cells straight into mature red blood cells. However that method presently produces small numbers of mature cells and requires repeat donations.
The team in Bristol have now developed a robust and reproducible technique which allows the production of immortalised erythroid cell lines from adult stem cells. These premature red cells can be cultured indefinitely, allowing larger-scale production, before being differentiated into mature red blood cells.
Dr Jan Frayne, from The University of Bristol School of Biochemistry, said: “Previous approaches to producing red blood cells have relied on various sources of stem cells which can only presently produce very limited quantities. By taking an alternative approach we have generated the first human immortalised adult erythroid line (Bristol Erythroid Line Adult or BEL-A), and in doing so, have demonstrated a feasible way to sustainably manufacture red cells for clinical use from in vitro culture.
“Globally, there is a need for an alternative red cell product. Cultured red blood cells have advantages over donor blood, such as reduced risk of infectious disease transmission.”
Prof Dave Anstee, director at the NIHR Blood and Transplant Research Unit in Red Cell Products, which is a collaboration between the University of Bristol and NHS Blood and Transplant, said: “The first therapeutic use of a cultured red cell product is likely to be for patients with rare blood groups because suitable conventional red blood cell donations can be difficult to source.
“The patients who stand to potentially benefit most are those with complex and life-limiting conditions like sickle cell disease and thalassemia, which can require multiple transfusions of well matched blood. The intention is not to replace blood donation but provide specialist treatment for specific patient groups.”
The cells were cultured at the University of Bristol and at NHS Blood and Transplant’s Filton site.
The research was funded by The Department of Health, The Wellcome Trust, NHS Blood and Transplant, BrisSynBio via a BBSRC/EPSRC Synthetic Biology Research Centre Grant, National Institute for Health Research Blood and Transplant Unit (NIHR BTRU) in Red Blood Cell Products at the University of Bristol in Partnership with NHS Blood and Transplant (NHSBT).
The research is available to view here.
